Composite

Part:BBa_K4059056

Designed by: Julie le-Fevre Hansen   Group: iGEM21_SDU-Denmark   (2021-10-21)


CPR from Phanerochaete chrysosporium with N-terminal deletion (12), E. coli K12 codon optimized, wit

CPR from Phanerochaete chrysosporium with N-terminal deletion (12), E. coli K12 codon optimized, with LacI

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 3628
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 3629
    Illegal PstI site found at 3643
    Illegal NotI site found at 1444
    Illegal NotI site found at 3636
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 3629
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 3629
    Illegal PstI site found at 3643
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None